Journal: Frontiers in Cell and Developmental Biology

Article Title: Multimodal profiling uncovers an aging-associated ETS1-MYO1B vascular niche responsive to ipragliflozin

doi: 10.3389/fcell.2026.1968248

Figure Lengend Snippet: MYO1B correlates with an aging-associated pericyte senescence subcluster (A) NMF consensus cluster heatmaps of pericytes in two datasets. (B) UMAP showing spatial distributions of pericyte clusters 1-3 in dataset 1 and dataset 2. (C) UMAP showing age distribution in the datasets. (D) Sample-proportion plots of clusters across age groups. (E) Heatmap of cluster markers in two datasets. (F) Violin plots of MYO1B expression across pericyte clusters 1-3. (G) Violin plots of MYO1B expression across age groups. (H) Representative H&E, MYO1B IHC, p21 IHC and PAI-1 IHC images from young and old skin samples.

Article Snippet: The antibody information was listed as followed MYO1B (Proteintech, 15012-1-AP), p21/CDKN1A (Proteintech, 10355-1-AP), PAI-1/SERPINE1 (Proteintech, 66261-1-Ig), PDGFRB antibody (Proteintech, 13449-1-AP) and ETS1 (Proteintech, 12118-1-AP).

Techniques: Expressing

Journal: Aging Cell

Article Title: SIRT1 Silences L1 Retrotransposons by Stabilizing Heterochromatin‐Modifying Complexes

doi: 10.1111/acel.70689

Figure Lengend Snippet: SIRT1 suppresses cellular senescence and this effect is rescued by 3TC treatment. (A) Schematic of induction in control, SIRT1‐overexpressing and SIRT1 knockdown cells post‐IR. (B) Western blot analysis of SIRT1 expression in HCA2‐hTERT cells. The HCA2‐hTERT cells were transfected with the control vector or the SIRT1 overexpression vector. (C) Quantification of SA‐β‐gal positive HCA2‐hTERT cells. HCA2‐hTERT cells were exposed to 10 Gy of X‐ray irradiation and transfected with the control vector or the SIRT1 overexpression vector for 7 days. Images from different areas of the plate were taken and quantified. (D) Representative images of SA‐β‐gal staining in HCA2‐hTERT cells, which were transfected with the control vector or the SIRT1 overexpression vector (Scale bar: 50 μm). (E) Western blot analysis of p21 and p16 in control and SIRT1‐overexpressing HCA2‐hTERT cells. (F) Western blot analysis of SIRT1 expression in control and SIRT1‐knockdown HCA2‐hTERT cells. (G) Western blot analysis of p21 and p16 in control and SIRT1‐knockdown HCA2‐hTERT cells. (H) Relative mRNA levels of CCL2 and MMP3 in control and SIRT1‐overexpressing HCA2‐hTERT cells. (I) Relative mRNA levels of CCL2 and MMP3 in control and SIRT1‐knockdown HCA2‐hTERT cells. The cells were treated with DMSO or 3TC. H, I: bars represent mean ± SD of biological replicates. Two‐tailed Student's t ‐test. C: bars represent mean ± SEM Mann–Whitney U test. Elements created with Figdraw.com .

Article Snippet: The antibodies employed in the study included anti‐SIRT1 (CST, Cat. #D739); anti‐KAP1 (ABclonal, Cat. #A19568), anti‐Lamin B1 (ABclonal, Cat. #A11495), anti‐p21 (ABclonal, Cat. #A19094), anti‐p16 (ABclonal, Cat. #A23882), anti‐TBK1 (CST, Cat. #3504), anti‐Phospho‐TBK1 (CST, Cat. #5483), anti‐STING (ABclonal, Cat. #A21051), anti‐Phospho‐STING (ABclonal, Cat. #AP1369), anti‐H3K9me3 (CST, Cat. #5326), anti‐Flag (ABclonal, Cat. #AE005), anti‐GFP (Abcam, Cat. #ab290), anti‐HA tag (CST, Cat. #3724), anti‐γH2AX (S139) (CST, Cat. #9718), anti‐Tubulin (ABclonal, Cat. #AC007), anti‐rabbit IgG‐HRP (Bio‐Rad, Cat. #170–6515), anti‐rabbit IgG H&L DyLight 488 (Abcam, Cat. #ab96899) and anti‐mouse IgG H&L DyLight 594 (Abcam, Cat. #ab96881).

Techniques: Control, Knockdown, Western Blot, Expressing, Transfection, Plasmid Preparation, Over Expression, Irradiation, Staining, Two Tailed Test, MANN-WHITNEY